Ashley Mila

Ashley Mila
Mentor: Dr. Mansour Mohamadzadeh
College of Medicine
 
"I am a student volunteer in the lab of Mansour Mohamadzadeh PhD. There, I learn about immunology and its roll in inflammation with IBD such as colon cancer, colitis, and crohn's disease. I am also being trained in lab techniques such as western blots, PCR, flow cytometry analysis, cell culture, and cellular staining. I first got involved in research because I find our ability to creatively think of ways to cure the human body amazing."

Major

Microbiology & Cell Science

Minor

N/A

Research Interests

  • Immunology
  • Dermatology
  • Inflammatory Bowel Disease

Academic Awards

  • Bright Futures
  • University Scholars

Organizations

  • Medical Journal Society
  • National Society of Collegiate Scholars
  • AMSA

Volunteer

  • PACE
  • AMSA
  • Global Brigades

Hobbies and Interests

  • Fitness / Zumba / Hip Hop
  • Cake Decorating
  • Research
  • Wakeboarding

Research Description

SignR3 Receptor Transfection in Macrophages, SlpA Induced Inflammatory Responses, and Proteobacteria's Role in Colitis Model

"I have been working with three experimental projects. Two of these projects are led by Bikash Sahay, MVSc, PhD, Tradib , PhD, and Yaima Lightfoot, PhD.

SignR3 Receptor Transfection in Macrophages: This project involves the transfection of a raw macrophage cell line with the signR3 plasmid. This way, Our raw macrophage cell line will express the SignR3 receptor and be susceptible to induction by slpA. In this Project I am assisted by Tradib ,PhD.

SlpA Induced Inflammatory Responses: This project involves inducing bone marrow derived dendritic cells and SignR3 transfected raw cells with slpA. SlpA is a surface protein on Lactobacillus Acidophilus, a common gut microbe. We will then analyze the cytokines for an inflammatory or anti-inflammatory response. In this project I am monitored by Bikash Sahay, MVSc, PhD.

Proteobacteria's Role in Murine Colitis Model: This project involves proteobacteria, a common bacteria found in the gut as well as in milk and cheese products. I assist Mansour Mohamadzadeh's team with this project. "