Diego Nolasco

Mentor: Dr. Georgiy Aslanidi
College of Medicine
 
"I have always been interested in science. Thus, when I arrived here at UF, I felt that doing research was a natural step for me. I get to apply concepts from multiple classes, which makes the learning process all the more rewarding. Furthermore, I get to know that the research I am a part of could possibly cure cancer in the near future. This is a very gratifying feeling. "

Major

Computer Science

Minor

N/A

Research Interests

  • Gene Therapy
  • Cancer
  • AAV Vectors

Academic Awards

  •  
  • College of Engineering Dean's List: 2012 - Present
  • University Scholars Program: 2014

Organizations

  • UF Programming Team
  • Streetlight
  • Donate Life

Volunteer

  • Streetlight
  • Top Soccer
  • GMS Community Outreach

Hobbies and Interests

  • Basketball
  • Film
  • Programming
  • Chess

Research Description

Development of Library of Adeno-Associated Virus Vectors for Cancer Gene Therapy.

In my project, we attempt to randomly combine all previously mutated 7 tyrosines, 15 serines and 17 threonines on a single viral capsid by the commonly used DNA shuffling technique. AAV capsid genes will randomly be fragmented by DNaseI followed by PCR amplification. During PCR, different single mutated capsid genes will be cross primed. This approach allows us to generate a limited library of viral capsids for future selection. These new AAV variants can then be tested for high transduction efficiency on various human cancer cell lines. The potential outcome of this project will be the creation of a superior vector for cancer gene therapy.