Lan Boi La
"University Scholar program provides me a unique opportunity to participate in research. I am able to research in my field of interest with this program. It gives me the opportunity to develop a proposal and bring my work to a presentable level. From this experience, I hope to become a competent undergraduate student in my area of study through research. I hope to gain research and presentation skills that are useful for my graduate studies. I hope to develop a research project that is contributable to the world of science."
Biochemistry, Nutritional Sciences
Molecular and oral biology.
Academic and Other Awards
- Godbold Foundation Scholarship
- Science for Life Intramural Research Award
- Florida Alliance Scholar
- Charles Vincent Heidi Cole McLaughlin Scholarship
- University Women's Club Scholarship
- Albert G. Raybun Scholarship
- Collegiate Health Service Corps
- Health Educated Asian Leaders
- Pre-Dental American Student Dental Association
Shands Hospital UF Office of Academic Support.
Hobbies and Interests
- Cooking, singing, arts and crafts, and swimming.
miR-146a and Other Innate Immune Related miRNAs in Generalized Severe Chronic Periodontitis
Periodontitis is a bacterial driven oral disease, associated with aggressive immune and inflammatory activation which results in the destruction of collagen and bone that support the teeth. Bacterial pathogens that induce periodontitis such as Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia have been shown in infected ApoE-/- mice and human THP-1 monocytes to affect miR-146a expression and mRNA expression for inflammatory cytokines TNF- α. Other innate immune related miRNAs that were detected include miR-132 and miR-155. More specifically, miR-146a has been demonstrated to regulate cytokine production in response to bacteria ligands and is correlated with TNF- α secretion, which reduces IRAK-1 and TRAF6 adaptor kinases. The upregulation of miR-146a in mouse periodontium and spleen during periodontal pathogen infection may contribute to controlling the onset of periodontal inflammation. RNA extraction from saliva has been made possible in a low-cost non-invasive screening assay. The extraction technique uses a combined protocol from Oragene RNA collection kit and the mirVana miRNA isolation kit in tandem to increase quality and yield of RNAs. These RNAs in the form of mRNAs and microRNAs (miRNAs) can serve as biomarkers reflective of diseases. To further investigate the expression of miR-146a and its mechanism in humans, RNA extraction based on a well-defined protocol in the laboratory can be utilized to isolate and identify miR-146a and other miRNAs in saliva of periodontal patients. Quantification of mature miRNAs can be performed using reverse transcription polymerase chain reaction and miRNA assay primers. The expression of miR-146a and other miRNAs have been correlated to periodontal diseases from the infection of specific bacterial pathogens in ApoE-/- mice. Therefore, by investigating the detailed mechanism, and function of miR-146a and other innate immune related miRNAs, better prognosis using saliva and possible treatments can be developed for such patients.