In my research, I study protein interactions in the adenovirus viral reproductive mechanism. The adenovirus goes through great lengths in order to prevent its host cell from accumulating p53 protein, or the "guardian angel" protein. This prevents the host cell from undergoing apoptosis and subsequently turns the cell into a viral protein factory. Many cancers are caused by an error in the p53 protein, and so studying this virus' reproductive pathway will give us insight as to how the cell cycle becomes deregulated via p53 inhibition. In my project, I hypothesized that viral E1A protein interaction with host factor p300/CBP is required for DBP expression. To test this, I will use a panel of E1A constructs including wild type and several mutants that do not bind to p300/CBP. I will carry out transfection experiments followed by immunofluorescence to analyze my results. The goal of my project is to further our understanding of how viral proteins utilize host factors for viral reproduction.